Device
Ptet-ChiXm

Part:BBa_K564020

Designed by: Anna Lewinska   Group: iGEM11_DTU-Denmark   (2011-09-20)

Mutated small RNA involved in RNA regulation of ChiP.

This device is based on gene encoding small RNA (sRNA)called ChiX, which represses ChiP gene synthesis by pairing with a sequence in 5’ untranslated region (UTR) near ribosome binding site (RBS) of chiP mRNA. This leads to degradation of the targeted mRNA by enzymes called RNases. The repression occurs in the absence of the substrates (chitooligosaccharides) stimulating chiP gene transcription. ChiX is fused with Ptet promoter. In this biobrick we introduced mutations in the ChiP and ChiXR binding site of ChiX in order to show that basepairing is essential for this regulatory system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None