Device
Pbad-ChiXR

Part:BBa_K564011

Designed by: Anna Lewinska   Group: iGEM11_DTU_Denmark   (2011-09-20)

ara-->chiXR (sRNA) device

ChiXR originates from the integenic region between genes chbB and chbC of enterobacteriacea. chiXR is a poorly defined sRNA that regulates the chiP gene through trapping the downregulating chiX sRNA. chiXR therefore has an indirect stimulatory effect on ChiP synthesis. The E. coli chiXR gene has been put under the control of the arabinose promoter in this biobrick so as to easily control its expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None