Measurement
Pbad-lacZ

Part:BBa_K564009

Designed by: Damian Rafal Plichta   Group: iGEM11_DTU_Denmark   (2011-09-20)


Arabinose promoter fused with lacZ

Para (PBAD) is an E. coli promoter that is induced by the presence of arabinose in the medium. In this part it is fused with lacZ which encodes β-galactosidase (an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose). This fusion allows us to check if Para works correctly using β-galactoassays.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None