Measurement
Ptet-lacZ

Part:BBa_K564008

Designed by: Damian Rafal Plichta   Group: iGEM11_DTU_Denmark   (2011-09-20)


Ptet fused with LacZ

Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog,aTc.. In this part it is fused with lacZ which encodes β-galactosidase (an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose). This fusion allows us to check if Ptet works correctly using β-galactoassays.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None