Composite
ChiP-lacZ

Part:BBa_K564005

Designed by: Anna Lewinska   Group: iGEM11_DTU-Denmark   (2011-09-19)


Chitoporin fused with lacZ -target for sRNA based regulation

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole sources of both carbon and nitrogen. It is only synthesized in the presence of these substrates.In nature it is subject of regulation by sRNAs.The gene is fused with lacZ to allow to check if the regulation works correctly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None