DNA
ChiP

Part:BBa_K564004

Designed by: Anna Lewinska   Group: iGEM11_DTU-Denmark   (2011-09-19)

ChiP promoter and 5' UTR; WT RBS (AAGAGG)

ChiP encodes an enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole sources of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is subject to post-transcriptional regulation by sRNA chiX (also known as micM or sroB) which pairs to its RBS region, preventing translation and triggering chiP mRNA destabilisation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None