Plasmid_Backbone

Part:BBa_K563053

Designed by: Guo Jing   Group: iGEM11_Tianjin   (2011-10-05)


vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

pYES2 is a 5.9 kb vector for inducible expression of recombinant proteins in S.cerevisivae. Feature of the vector allow easy cloning of your gene of interest and selection of transformants by uracil prototrophy. The vector contains the follow elements:

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 595
    Illegal SpeI site found at 525
    Illegal SpeI site found at 5851
    Illegal PstI site found at 555
    Illegal PstI site found at 3632
    Illegal PstI site found at 4120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal NheI site found at 3839
    Illegal SpeI site found at 525
    Illegal SpeI site found at 5851
    Illegal PstI site found at 555
    Illegal PstI site found at 3632
    Illegal PstI site found at 4120
    Illegal NotI site found at 576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal BamHI site found at 519
    Illegal XhoI site found at 583
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 595
    Illegal SpeI site found at 525
    Illegal SpeI site found at 5851
    Illegal PstI site found at 555
    Illegal PstI site found at 3632
    Illegal PstI site found at 4120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 595
    Illegal SpeI site found at 525
    Illegal SpeI site found at 5851
    Illegal PstI site found at 555
    Illegal PstI site found at 3632
    Illegal PstI site found at 4120
    Illegal NgoMIV site found at 5709
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1996
    Illegal BsaI.rc site found at 2942
    Illegal SapI site found at 913
    Illegal SapI site found at 3094


[edit]
Categories
Parameters
n/avector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.