Part:BBa_K563037

pEASY+33Tor2(with E->K mutation)

There are totally four mutation sites as we designed in the complete Tor2 sequence. This BioBrick contains the third part of Tor2 with mutation that from amino acid E to amino acid K.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 910
  Illegal XbaI site found at 906
  Illegal XbaI site found at 1736
  Illegal XbaI site found at 2843
  Illegal SpeI site found at 258
  Illegal SpeI site found at 1688
  Illegal PstI site found at 2803
  Illegal PstI site found at 3993
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 910
  Illegal NheI site found at 2048
  Illegal SpeI site found at 258
  Illegal SpeI site found at 1688
  Illegal PstI site found at 2803
  Illegal PstI site found at 3993
  Illegal NotI site found at 2824
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 910
  Illegal BglII site found at 1970
  Illegal BglII site found at 3780
  Illegal BamHI site found at 252
  Illegal BamHI site found at 317
  Illegal XhoI site found at 2831
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 910
  Illegal XbaI site found at 906
  Illegal XbaI site found at 1736
  Illegal XbaI site found at 2843
  Illegal SpeI site found at 258
  Illegal SpeI site found at 1688
  Illegal PstI site found at 2803
  Illegal PstI site found at 3993
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 910
  Illegal XbaI site found at 906
  Illegal XbaI site found at 1736
  Illegal XbaI site found at 2843
  Illegal SpeI site found at 258
  Illegal SpeI site found at 1688
  Illegal PstI site found at 2803
  Illegal PstI site found at 3993
  Illegal NgoMIV site found at 3169
  Illegal NgoMIV site found at 4444
  Illegal AgeI site found at 2067
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5343
  Illegal SapI.rc site found at 4293
  Illegal SapI.rc site found at 4503