Coding
Part:BBa_K563036
Designed by: Guo Jing Group: iGEM11_Tianjin (2011-10-05)
pEASY+33Tor2(with V->A mutation)
There are totally four mutation sites as we designed in the complete Tor2 sequence. This BioBrick contains the third part of Tor2 with mutation that from amino acid V to amino acid A.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 910
Illegal XbaI site found at 906
Illegal XbaI site found at 1736
Illegal XbaI site found at 2843
Illegal SpeI site found at 258
Illegal SpeI site found at 1688
Illegal PstI site found at 2803
Illegal PstI site found at 3993 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 910
Illegal NheI site found at 2048
Illegal SpeI site found at 258
Illegal SpeI site found at 1688
Illegal PstI site found at 2803
Illegal PstI site found at 3993
Illegal NotI site found at 2824 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 910
Illegal BglII site found at 1970
Illegal BglII site found at 3780
Illegal BamHI site found at 252
Illegal BamHI site found at 317
Illegal XhoI site found at 2831 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 910
Illegal XbaI site found at 906
Illegal XbaI site found at 1736
Illegal XbaI site found at 2843
Illegal SpeI site found at 258
Illegal SpeI site found at 1688
Illegal PstI site found at 2803
Illegal PstI site found at 3993 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 910
Illegal XbaI site found at 906
Illegal XbaI site found at 1736
Illegal XbaI site found at 2843
Illegal SpeI site found at 258
Illegal SpeI site found at 1688
Illegal PstI site found at 2803
Illegal PstI site found at 3993
Illegal NgoMIV site found at 3169
Illegal NgoMIV site found at 4444
Illegal AgeI site found at 2067 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5343
Illegal SapI.rc site found at 4293
Illegal SapI.rc site found at 4503
[edit]
Categories
Parameters
n/a | pEASY+33Tor2(with V->A mutation) |