Coding
Part:BBa_K563032
Designed by: Guo Jing Group: iGEM11_Tianjin (2011-10-05)
pEASY with second part of Tor2
We separate Tor2 into three parts, and the first part is called 01Tor2, including the promoter. pEASY are blunt vector provided by transgene. We link 01Tor2 with this vector for further connection.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2005
Illegal EcoRI site found at 2425
Illegal EcoRI site found at 2708
Illegal XbaI site found at 1158
Illegal XbaI site found at 2965
Illegal XbaI site found at 3053
Illegal SpeI site found at 258
Illegal PstI site found at 3013
Illegal PstI site found at 4203 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2005
Illegal EcoRI site found at 2425
Illegal EcoRI site found at 2708
Illegal NheI site found at 2805
Illegal SpeI site found at 258
Illegal PstI site found at 3013
Illegal PstI site found at 4203
Illegal NotI site found at 3034 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2005
Illegal EcoRI site found at 2425
Illegal EcoRI site found at 2708
Illegal BglII site found at 3990
Illegal BamHI site found at 252
Illegal BamHI site found at 2991
Illegal XhoI site found at 3041 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2005
Illegal EcoRI site found at 2425
Illegal EcoRI site found at 2708
Illegal XbaI site found at 1158
Illegal XbaI site found at 2965
Illegal XbaI site found at 3053
Illegal SpeI site found at 258
Illegal PstI site found at 3013
Illegal PstI site found at 4203 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2005
Illegal EcoRI site found at 2425
Illegal EcoRI site found at 2708
Illegal XbaI site found at 1158
Illegal XbaI site found at 2965
Illegal XbaI site found at 3053
Illegal SpeI site found at 258
Illegal PstI site found at 3013
Illegal PstI site found at 4203
Illegal NgoMIV site found at 2344
Illegal NgoMIV site found at 3379
Illegal NgoMIV site found at 4654
Illegal AgeI site found at 1584 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5553
Illegal SapI.rc site found at 2014
Illegal SapI.rc site found at 2266
Illegal SapI.rc site found at 4503
Illegal SapI.rc site found at 4713
[edit]
Categories
Parameters
n/a | pEASY with second part of Tor2 |