Coding

Part:BBa_K563032

Designed by: Guo Jing   Group: iGEM11_Tianjin   (2011-10-05)


pEASY with second part of Tor2

We separate Tor2 into three parts, and the first part is called 01Tor2, including the promoter. pEASY are blunt vector provided by transgene. We link 01Tor2 with this vector for further connection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2005
    Illegal EcoRI site found at 2425
    Illegal EcoRI site found at 2708
    Illegal XbaI site found at 1158
    Illegal XbaI site found at 2965
    Illegal XbaI site found at 3053
    Illegal SpeI site found at 258
    Illegal PstI site found at 3013
    Illegal PstI site found at 4203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2005
    Illegal EcoRI site found at 2425
    Illegal EcoRI site found at 2708
    Illegal NheI site found at 2805
    Illegal SpeI site found at 258
    Illegal PstI site found at 3013
    Illegal PstI site found at 4203
    Illegal NotI site found at 3034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2005
    Illegal EcoRI site found at 2425
    Illegal EcoRI site found at 2708
    Illegal BglII site found at 3990
    Illegal BamHI site found at 252
    Illegal BamHI site found at 2991
    Illegal XhoI site found at 3041
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2005
    Illegal EcoRI site found at 2425
    Illegal EcoRI site found at 2708
    Illegal XbaI site found at 1158
    Illegal XbaI site found at 2965
    Illegal XbaI site found at 3053
    Illegal SpeI site found at 258
    Illegal PstI site found at 3013
    Illegal PstI site found at 4203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2005
    Illegal EcoRI site found at 2425
    Illegal EcoRI site found at 2708
    Illegal XbaI site found at 1158
    Illegal XbaI site found at 2965
    Illegal XbaI site found at 3053
    Illegal SpeI site found at 258
    Illegal PstI site found at 3013
    Illegal PstI site found at 4203
    Illegal NgoMIV site found at 2344
    Illegal NgoMIV site found at 3379
    Illegal NgoMIV site found at 4654
    Illegal AgeI site found at 1584
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5553
    Illegal SapI.rc site found at 2014
    Illegal SapI.rc site found at 2266
    Illegal SapI.rc site found at 4503
    Illegal SapI.rc site found at 4713


[edit]
Categories
Parameters
n/apEASY with second part of Tor2