Regulatory

Part:BBa_K563002

Designed by: Boxuan Zeng   Group: iGEM11_Tianjin   (2011-10-05)


promoter of ILV2

Promoter of ILV2, under control of transcription factor Gcn4. In our project, when When TORC1 is inhibited by FAP, dephosphorylated Gcn4 would bind with pHIS4 and activate downstream genes. We put the target gene of mutant Tor2 under this promoter, and then its expression could be controlled by the amount of FAP and the activity of TORC1. Mutant Tor2 would be much resistant to FAP and a feedback loop is formed between FAP, TORC1, Gcn4 and mTor.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 376
    Illegal SpeI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 376
    Illegal SpeI site found at 395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 376
    Illegal BglII site found at 319
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 376
    Illegal SpeI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 376
    Illegal SpeI site found at 395
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apromoter of ILV2