Regulatory

Part:BBa_K563001

Designed by: Saisi Xue   Group: iGEM11_Tianjin   (2011-09-27)

pDAL7(malate synthase promoter)

DAL7 encodes malate synthase in yeast cell, whose expression is sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation. Evidence showed that DAL7 had transcriptional response to nitrogen availability through two transcription factors Gln3 and Gat1, for the expression of DAL7 is greatly induced in the gln3Δ–gat1Δ strain. Therefore it is predicted that DAL7 is under control of a promoter which is sensitive to regulators Gln3 and Gat1. When TORC1 (TOR Complex1) is inhibited by FAP, dephosphorylated Gln3 and Gat1 would bind with pDAL7 and activate downstream genes. We put the target gene of mutant Tor2 under this promoter, and then its expression could be controlled by the amount of FAP and the activity of TORC1. Mutant Tor2 would be much resistant to FAP and a feedback loop is formed between FAP, TORC1, Gln3, Gat1 and mTor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 510
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
n/apDAL7(malate synthase promoter)