Regulatory

Part:BBa_K563000

Designed by: Saisi Xue   Group: iGEM11_Tianjin   (2011-09-27)


pHIS4 (Multifunctional Histidine biosynthesis enzyme promoter)

HIS4 encodes a multifunctional polypeptide that has phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities which can catalyze histidine biosynthesis in yeast. Transcription of HIS4 is regulated by general amino acid control, in which the transcription factor Gcn4p plays a key role. Therefore it is predicted that His4 is under control of a promoter which is sensitive to transcription factor Gcn4p. When TORC1 is inhibited by FAP, dephosphorylated Gcn4 would bind with pHIS4 and activate downstream genes. We put the target gene of mutant Tor2 under this promoter, and then its expression could be controlled by the amount of FAP and the activity of TORC1. Mutant Tor2 would be much resistant to FAP and a feedback loop is formed between FAP, TORC1, Gcn4 and mTor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 520
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
n/apHIS4 (Multifunctional Histidine biosynthesis enzyme promoter)