Coding
PduD40-GFP

Part:BBa_K562019

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-18)


Salty_PduD40-GFPssrA

This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to GFP. The GFP gene product also carries a C-terminal ssrA degradation tag. Production of PduD40-GFP-ssrA has been verified by Western immunoblotting (anti-GFP) when co-expressed with BBa_K562009 encosing a bacterial microcompartment (BMC). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-GFPssrA in the Sargent Laboratory, Dundee, UK.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 239
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 239
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 239
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 885


[edit]
Categories
Parameters
None