Coding
PduD40-Asp
Part:BBa_K562017
Designed by: Frank Sargent Group: iGEM11_Dundee (2011-09-18)
Salty_PduD40-Asp
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to L-aspartate ammonia-lyase (AspA) from Escherichia coli (from part BBa_C0083). The AspA gene product also carries a C-terminal HA epitope tag. Production of PduD20-mCherry has been verified by Western immunoblotting (anti-HA). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-Asp in the Sargent Laboratory, Dundee, UK.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 239
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 313
Illegal BamHI site found at 1784 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 239
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 239
Illegal NgoMIV site found at 1205
Illegal AgeI site found at 734
Illegal AgeI site found at 1586 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1245
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Categories
Parameters
None |