Coding
PduD40-Xyl

Part:BBa_K562016

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-18)


Salty_PduD40-XylA

This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to D-xylose isomerase (XylA) from Escherichia coli K-12. The XylA gene product also carries a C-terminal HA epitope tag. Production of PduD20-XylA has been verified by Western immunoblotting (anti-HA). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-XylA in the Sargent Laboratory, Dundee, UK.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 239
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1617
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 239
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 239
    Illegal AgeI site found at 1238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 356


[edit]
Categories
Parameters
None