Part:BBa_K562005
Salty_PduN BMC Component
This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the PduN protein from Salmonella enterica serovar Typhimurium LT2. This is a component of the Salmonella bacterial microcompartment (BMC). The PduN gene product carries a C-terminal 6-His affinity/epitope tag. Production of the PduN protein has been verified by Western immunoblotting (anti-penta-His) and production of PduN has been confirmed by 35S-Met labelling. The protein has also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-N in the Sargent Laboratory, Dundee, UK.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 413
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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