Tag
Salty_D40

Part:BBa_K562002

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-16)


Salty_PduD40 Targeting Tag

This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the first 40 amino acid residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2. This is a targeting sequence for the Salmonella Pdu bacterial microcompartment (BMC). This 40 residue targeting sequence has been tested by fusion to GFP and it does work. The construct is coloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40 in the Sargent Laboratory, Dundee, UK.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 239
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 239
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 239
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None