Device

Part:BBa_K561004

Designed by: LI Ye, XIA Yu   Group: iGEM11_ZJU-China   (2011-10-03)

PfdhF+cex+cenA+tetR

Bottom Layer of the SugarFilm expression system aimed for cellulose degradation. Exoglucanase (Cex) and endoglucanase A (CenA) coding sequence from Cellulomonas fimi (iGEM08_Edinburgh) are under the regulation of anaerobic promoter fdhF. The expression of tetR will repress Ptet, which is in the device for upper-layer expression in the biofilm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 649
    Illegal NotI site found at 1646
    Illegal NotI site found at 2790
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2703
    Illegal BamHI site found at 1839
    Illegal XhoI site found at 2201
    Illegal XhoI site found at 2450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 282
    Illegal NgoMIV site found at 655
    Illegal NgoMIV site found at 1157
    Illegal NgoMIV site found at 1981
    Illegal NgoMIV site found at 2906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1885
    Illegal BsaI.rc site found at 702
    Illegal SapI.rc site found at 785


[edit]
Categories
Parameters
None