Part:BBa_K560016

BsaI Golden Gate Assembly_Backbone

The backbone for golden gate assembly. This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors(BBa_K560012, BBa_K560013, BBa_K560014, BBa_K560015), and add adequate amount of ligase and Bsal. More details about experiment protocol can be found in our wiki. Details about the structure of this plasmid can be found in http://www.addgene.org/31021/.

The Genetic Map

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 644
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NheI site found at 35
  Illegal NheI site found at 2776
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XhoI site found at 363
  Illegal XhoI site found at 1797
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 644
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 644
  Illegal NgoMIV site found at 1650
  Illegal AgeI site found at 569
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 575
  Illegal BsaI.rc site found at 128
  Illegal SapI.rc site found at 2653