Coding
ThiE

Part:BBa_K558009

Designed by: Laura Colgin, Megan Tabor   Group: iGEM11_Nevada   (2011-09-23)

Thiamin monophosphate pyrophosphorylase

This is the coding sequence of thiamin monophosphate pyrophosphorylase (ThiE) from the unicellular cyanobacterium Synechocystis sp. PCC 6803. It encodes 2-[3-[(4-Amino-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-thiazol-5-yl] ethanol. By placing overexpression gene cassettes into the coding region of ThiE an auxotophic mutant that can only survive with thiamin (Vitamin B1) supplementation can be created.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 326
    Illegal BsaI.rc site found at 967


[edit]
Categories
//chassis/prokaryote/cyanobacterium
//chassis/prokaryote/synechocystis
//function/dna
Parameters
biology
genotype