Coding

Part:BBa_K558008

Designed by: Megan Tabor   Group: iGEM11_Nevada   (2011-09-22)

Bay Laurel Thioesterase coding region

Bay Laurel Thioesterase coding region Bay Laurel Thioesterase (BTE) will intercept acyl-ACP from the fatty acid synthesis cycle and turn it into the fatty acid derivatives C12 and C14.

L Yuan, T A Voelker, and D J Hawkins, (1995), Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. Proc Natl Acad Sci U S A. November 7; 92(23): 10639–10643.
Welch et. al. “Design Parameters to Control Synthetic Gene Expression in Escherichia coli”


UNRFfa_pathway.jpg


UNRBTE_coding_construct.jpg

Bay Laurel Thioesterase has been shown to produce free fatty acids in part BBa K558008

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 302
    Illegal BsaI.rc site found at 712


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Categories
Parameters
None