Intermediate
BTE

Part:BBa_K558003

Designed by: Megan Tabor   Group: iGEM11_Nevada   (2011-09-07)

Bay Laurel Thioesterase (RBS Terminator)

Bay Laurel Thioesterase with a RBS, and the double terminator (BBa_B0014) Bay Laurel Thioesterase (BTE) will intercept acyl-ACP from the fatty acid synthesis cycle and turn it into the fatty acid derivatives C12 and C14.

L Yuan, T A Voelker, and D J Hawkins, (1995), Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. Proc Natl Acad Sci U S A. November 7; 92(23): 10639–10643.
Welch et. al. “Design Parameters to Control Synthetic Gene Expression in Escherichia coli”


UNRFfa_pathway.jpg


UNRBTE_inter_construct.jpg

Bay Laurel Thioesterase has been shown to work in BioBrick part BBa K558007

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 319
    Illegal BsaI.rc site found at 729


[edit]
Categories
Parameters
None