Intermediate

Part:BBa_K558000

Designed by: Jovanna Casas   Group: iGEM11_Nevada   (2011-08-25)

PDC/ADH coding regions

UNRPDCADH_construct1.jpg

This intermediate part (along with a promoter) was designed for ethanol production. This part includes the coding regions for Pyruvate Decarboxylase (PDC) and Alcohol Dehydrogenase (ADH) based on Zymomonas mobilis along with a standard E. coli ribosome binding site and double terminator(BBa_B0014).


The pyruvate decarboxylase gene converts pyruvate to acetaldehyde. Acetaldehyde is then converted to ethanol by alcohol dehydrogenase.

Pathway1unr.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1031
    Illegal AgeI site found at 1178
    Illegal AgeI site found at 1556
    Illegal AgeI site found at 2327
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 65
    Illegal BsaI site found at 2678
    Illegal SapI site found at 479
    Illegal SapI site found at 1142


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Parameters
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