Device

Part:BBa_K554016

Designed by: UNICAMP EMSE Brazil team   Group: iGEM11_UNICAMP-EMSE_Brazil   (2011-09-26)

flhDC GFP HlyA device

flhDC GFP HlyA device is part of the Device 1 testing: SoxR/SoxS system regulating GFP production. It is composed by flhDC promoter linked to RBS, GFP gene, HlyA and Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 735


Usage and Biology

In order to test the ability of Jedi Bacteria in sensing Catecholamine and AI-3 levels and activating genes linked to flhDC promoter (via QseC and QseB), we built a Device testing, with GFP linked to flhDC promoter, as it is shown in the following schema (Figure 1):

UNICAMP EMSE GFP flhdc device.jpg
Figure 1: Testing [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_1 Device 1] through replacement of IL-12 to GFP.
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Categories
Parameters
None