Device

Part:BBa_K554014

Designed by: UNICAMP EMSE Brazil team   Group: iGEM11_UNICAMP-EMSE_Brazil   (2011-09-26)

flhDC IL12 HlyA device

flhDC IL12 HlyA device is is used by [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil UNICAMP-EMSE Brazil team] in the [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_1:_Adrenaline_sensor.2FIL-12_producer CA sensor device/ IL-12 producer] ("Device 1", which senses Catecholamine levels (such as Adrenaline, Norepinephrine and AI-3) and responds by producing and secreting IL-12). This device is composed by flhDC promoter linked to RBS+Il-12 gene, HlyA and Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Schema:

This device is used by [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil UNICAMP-EMSE Brazil team] in the [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project/Device1 Adrenaline sensor device] ("Device 1", which senses Cathecolamine and AI-3 levels and responds by producing and secreting IL-12. This part is shown in red in the following schema:

UNICAMP EMSE IL 12 device.jpg
[edit]
Categories
Parameters
None