Regulatory
flhDC

Part:BBa_K554001

Designed by: UNICAMP EMSE Brazil team   Group: iGEM11_Brazil-France   (2011-09-21)

flhDC promoter

flhDC promoter is a regulatory sequence found upstream the flhDC genes in enterohaemorragic 'E. coli' (EHEC) and other pathogenic enterobacteria. Since flhDC are the master activators of the flagella regulon, (Clarke et al, 2005), therefore important to activate flagella and motility of such pathogens. Sperandio et al (2003) demonstrated that virulence genes and motility related genes such as flhDC are induced by the signals AI-3, epinephrine and norepinephrine through activation of QseC (a bacterial adrenergic receptor), which induces phosphorylation of the transcription factor QseB, which binds to flhDC promoter and activated transcription. Importantly, this interaction is dependent on QseB's phosphorylation state (Clarke et al, 2005). Only in the presence of catecholamines such as Adrenaline and Epinephrine, and the quorum-sensing auto inducer 3 the QseB will be phosphorylated by active form of QseC adrenergic receptor, which has a histidine sensor kinase domain (SPERANDIO et al. , 2002).

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

flhDC promoter is used by UNICAMP-EMSE Brazil team in the Adrenaline sensor device ("Device 1", which senses Cathecolamine and AI-3 levels and responds by producing and secreting IL-12. The flhDC is essential to the functioning of this device, since it is responsible for the response induction, being the IL-12 synthesis trigger.

This part is shown in purple in the following schema:

UNICAMP EMSE IL 12 device.jpg

References

  1. Clarke MB, Sperandio V (2005) Transcriptional regulation of flhDC by QseBC and sigma (FliA) in enterohaemorrhagic Escherichia coli. Mol Microbiol 57: 1734–1749. Link to PubMed
  2. Sperandio V, Torres AG, Jarvis B, Nataro JP, Kaper JB (2003) Bacteria-host communication: the language of hormones. Proc Natl Acad Sci USA 100: 8951–8956. Link to PubMed
  3. Sperandio V, Torres AG, Kaper JB (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol Microbiol 43: 809–821. Link to PubMed
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