Composite

Part:BBa_K553006

Designed by: Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto   Group: iGEM11_UNITS_Trieste   (2011-09-17)

C. firmi beta-glucosidase PlasB regulated

BBa_K392008 Beta-glucosidase expression under PLasB promoter.

The promoter is actively regulated by the LasR - OC12 HLA complex.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1257
    Illegal XhoI site found at 1490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 746
    Illegal AgeI site found at 189
    Illegal AgeI site found at 248
    Illegal AgeI site found at 642
    Illegal AgeI site found at 837
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 619


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