Composite

Part:BBa_K552004

Designed by: Jonathan Herrmann   Group: iGEM11_WashU   (2011-09-28)


CCD1 Gene-Codon Optimized for Yeast

This DNA sequence codes for the enzyme CCD1, also known as carotenoid cleavage dioxygenase 1. CCD1 cleaves beta-caroten to form two molecules of beta-ionone. This enzyme completes the beta-ionone biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
    Illegal XbaI site found at 735
    Illegal XbaI site found at 753
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
    Illegal XbaI site found at 735
    Illegal XbaI site found at 753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 771
    Illegal XbaI site found at 735
    Illegal XbaI site found at 753
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None