Composite

Part:BBa_K552003

Designed by: Jonathan Herrmann   Group: iGEM11_WashU   (2011-09-28)


CrtI Gene-Codon Optimized for Yeast

This DNA sequence codes for the enzyme CrtI, also known as phytoene desaturase. CrtI converts pytoene to lycopene which is an important step in the carotenoid biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1746
    Illegal XbaI site found at 1527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1746
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1746
    Illegal BglII site found at 1323
    Illegal BglII site found at 1944
    Illegal BglII site found at 2103
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1746
    Illegal XbaI site found at 1527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1746
    Illegal XbaI site found at 1527
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None