Composite

Part:BBa_K552001

Designed by: Jonathan Herrmann   Group: iGEM11_WashU   (2011-09-24)


CrtE Gene-Codon Optimized for Yeast

This DNA sequence codes for the enzyme CrtE, also known as geranylgeranyl diphosphate synthase. CrtE serves as the first step in carotenoid biosynthesis in Saccharomyces cerevisiae by converting farnesyl diphosphate (which is naturally found in yeast) to geranylgeranyl diphosphate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
    Illegal XbaI site found at 1229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
    Illegal BglII site found at 491
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
    Illegal XbaI site found at 1229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
    Illegal XbaI site found at 1229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 727


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None