Regulatory

Part:BBa_K549038

Designed by: Jara Radeck   Group: iGEM11_LMU-Munich   (2011-09-21)


Promotor prcnA of rcnA with RcnR-binding site and 50nt rcnA

This is the promoter of rcnA, called prcnA. It has a binding site for the regulator RcnR, which originates in E. coli. In presence of Ni(II), Ni(II) binds to RcnR, RcnR binds to the promoter and activates transcription. Co(II) can also be a stimulus, but a weaker one. This promoter contains the first ca. 50 nt of rcnA, and therefore is used for transcriptional fusion with genes with RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None