Measurement

Part:BBa_K549037

Designed by: Tobias Bauer   Group: iGEM11_LMU-Munich   (2011-09-16)


B. subtilis ase promoter with aseR + GFP reporter gene

The promotor PcueR is regulated by the repressor cueR. In presence of copper(2+) the reporter gene can be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 203
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 203
    Illegal AgeI site found at 855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1374


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Categories
Parameters
None