Measurement

Part:BBa_K549035

Designed by: Tobias Bauer   Group: iGEM11_LMU-Munich   (2011-09-16)


B. subtilis cue promoter with cueR + GFP reporter gene

The promotor PcueR is regulated by the repressor cueR. In presence of copper(2+) the reporter gene can be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 203
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 969


[edit]
Categories
Parameters
None