Measurement

Part:BBa_K549026

Designed by: Franziska Haefele   Group: iGEM11_LMU-Munich   (2011-09-16)


petE2 with the fused 10x His-tag

The BioBrick contains the gene petE2 with a C-terminal 10x His-tag. The backbone is the expression vector J04500.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 519
    Illegal PstI site found at 167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 167
    Illegal NotI site found at 510
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 365
    Illegal BglII site found at 384
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 519
    Illegal PstI site found at 167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 519
    Illegal PstI site found at 167
    Illegal NgoMIV site found at 527
    Illegal AgeI site found at 563
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None