Measurement

Part:BBa_K549022

Designed by: Franziska Haefele   Group: iGEM11_LMU-Munich   (2011-09-16)


pabA with fused His-tag

This part contains the pabA gene from the BioBrick K549020 with the C-terminal fused 10x His-tag from the BioBrick K549019. This construct is integrated into the BioBrick J04500 containing a constitutive promoter and RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 597
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 588
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 597
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 597
    Illegal NgoMIV site found at 605
    Illegal AgeI site found at 641
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None