Regulatory

Part:BBa_K546547

Designed by: Brendan Ryback, Matthijn Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-16)

Constitutive (tetR repressible) LacI and RFP expression

This part constitutively expresses LacI and RFP except when repressed by TetR. When cells containing this part are exposed to the inducer molecule IPTG, it can be used to regulate lac-repressible promoters. It was designed to function as an IPTG-controllable tuner for the Double Tunable Synchronized Oscillator. It is advisable to operate this part on a low or medium copy plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter/constitutive
//function/reporter/fluorescence
Parameters
control(1) R0040
directionForward
protein(1) LacI (2) RFP