Signalling

Part:BBa_K546003

Designed by: Brendan Ryback, Matthijn Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)

Lux pL controlled LuxR + lux pR promoter.

This part was made as a replacement for a defective version of BBa_F2621. After sequencing we noticed that BBa_F2621 was lacking the BBa_R0063 promoter. We tested the expression levels of BBa_K546003 and BBa_F2621 using RBS-J04031-B0015 as a reporter (See BBA_K546002). BBa_K546003 had a significantly higher GFP signal than the F2621 BioBrick part we received, this indicated that we fixed it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101

Experimental data

See K546002


[edit]
Categories
//classic/signalling/receiver
//function/cellsignalling
Parameters
controlR0063
device_typeReceiver
family
latencySeconds
proteinsLuxR
signalling_molecule3OC6HSL
switch_point2nM