Part:BBa_K5452134
Overexpression GPPS
this composite is responsible for expressing GPPS through a strong promoter, with the aim of generating greater production of GPP. Composite contains parts BBa_K801011,BBa_K5452107, BBa_J435219, BBa_K5452108. This composite CDS is for use in eukaryotic chassis, and is currently optimized for Saccharomyces cerevisiae. Assembled with no scars, suited for cloning and assembling into plasmids and other parts through Gibson Assembly.
Usage and Biology
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2812
Illegal EcoRI site found at 4495
Illegal XbaI site found at 4844
Illegal SpeI site found at 1474
Illegal SpeI site found at 5020
Illegal PstI site found at 1161
Illegal PstI site found at 3232 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2812
Illegal EcoRI site found at 4495
Illegal SpeI site found at 1474
Illegal SpeI site found at 5020
Illegal PstI site found at 1161
Illegal PstI site found at 3232
Illegal NotI site found at 2278 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2812
Illegal EcoRI site found at 4495
Illegal BamHI site found at 1254 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2812
Illegal EcoRI site found at 4495
Illegal XbaI site found at 4844
Illegal SpeI site found at 1474
Illegal SpeI site found at 5020
Illegal PstI site found at 1161
Illegal PstI site found at 3232 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2812
Illegal EcoRI site found at 4495
Illegal XbaI site found at 4844
Illegal SpeI site found at 1474
Illegal SpeI site found at 5020
Illegal PstI site found at 1161
Illegal PstI site found at 3232
Illegal AgeI site found at 106 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3922
Illegal SapI.rc site found at 278
Illegal SapI.rc site found at 1246
Illegal SapI.rc site found at 3769
Geranyl diphosphate synthase is the enzyme responsible for the conversion of IPP into GPP in the monoterpenoid pathway in S. cerevisiae. GPP is produced exclusively as an intermediate in the synthesis of farnesyl diphosphate, which results in very limited availability of GPP for monoterpene synthesis. However, the impact of GPP pool regulation in S. cerevisiae has not yet been extensively studied. Thus, the overexpression of GPPS in yeast could allow us to both increase GPP production and gain a better understanding of its functioning, which would be useful in various fields.
None |