Part:BBa_K5452133

RISC casette

Long description:This composite is responsible for coding the two enzymes present in the RISC system that act on the RNA interference mechanisms: Argonaute and Dicer. These parts are interspersed with the IRES ribosomal binding site. Composite contains parts BBa_K5452101,BBa_K5452102, BBa_K124002, BBa_K1993016, BBa_K5452105 BBa_K5452106, BBa_K2637017 This composite CDS is for use in eukaryotic chassis, and is currently optimized for Saccharomyces cerevisiae. Assembled with no scars, suited for cloning and assembling into plasmids and other parts through Gibson Assembly.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 6555
  Illegal PstI site found at 1946
  Illegal PstI site found at 4982
  Illegal PstI site found at 6829
  Illegal PstI site found at 8619
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1946
  Illegal PstI site found at 4982
  Illegal PstI site found at 6829
  Illegal PstI site found at 8619
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 7736
  Illegal BglII site found at 8074
  Illegal BamHI site found at 86
  Illegal BamHI site found at 2451
  Illegal XhoI site found at 595
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 6555
  Illegal PstI site found at 1946
  Illegal PstI site found at 4982
  Illegal PstI site found at 6829
  Illegal PstI site found at 8619
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 6555
  Illegal PstI site found at 1946
  Illegal PstI site found at 4982
  Illegal PstI site found at 6829
  Illegal PstI site found at 8619
  Illegal NgoMIV site found at 10394
  Illegal AgeI site found at 7521
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 9309
  Illegal BsaI site found at 9948
  Illegal SapI.rc site found at 9156