Device

Part:BBa_K5452128

Designed by: Bianca Henriques de Oliveira   Group: iGEM24_USP-EEL-Brazil   (2024-09-28)


RNAi cassette

This composite is a device capable of expressing double-stranded RNA based on a fragment of ERG20, which is recognized by the RISC system, inducing gene silencing. This composite contains parts BBa_K5452103, BBa_K5452100, BBa_K2601000, BBa_K5452104. This device is for use in eukaryotic chassis, and is optimized for Saccharomyces cerevisiae. Assembled with no scars, suited for cloning and assembling into plasmids and other parts through Gibson Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5101
    Illegal EcoRI site found at 5542
    Illegal EcoRI site found at 5973
    Illegal EcoRI site found at 6414
    Illegal XbaI site found at 1
    Illegal SpeI site found at 266
    Illegal PstI site found at 3968
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5101
    Illegal EcoRI site found at 5542
    Illegal EcoRI site found at 5973
    Illegal EcoRI site found at 6414
    Illegal SpeI site found at 266
    Illegal PstI site found at 3968
    Illegal NotI site found at 2797
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5101
    Illegal EcoRI site found at 5542
    Illegal EcoRI site found at 5973
    Illegal EcoRI site found at 6414
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5101
    Illegal EcoRI site found at 5542
    Illegal EcoRI site found at 5973
    Illegal EcoRI site found at 6414
    Illegal XbaI site found at 1
    Illegal SpeI site found at 266
    Illegal PstI site found at 3968
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5101
    Illegal EcoRI site found at 5542
    Illegal EcoRI site found at 5973
    Illegal EcoRI site found at 6414
    Illegal XbaI site found at 1
    Illegal SpeI site found at 266
    Illegal PstI site found at 3968
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4658
    Illegal SapI.rc site found at 4505


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Categories
Parameters
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