Device
Part:BBa_K5452128
Designed by: Bianca Henriques de Oliveira Group: iGEM24_USP-EEL-Brazil (2024-09-28)
RNAi cassette
This composite is a device capable of expressing double-stranded RNA based on a fragment of ERG20, which is recognized by the RISC system, inducing gene silencing. This composite contains parts BBa_K5452103, BBa_K5452100, BBa_K2601000, BBa_K5452104. This device is for use in eukaryotic chassis, and is optimized for Saccharomyces cerevisiae. Assembled with no scars, suited for cloning and assembling into plasmids and other parts through Gibson Assembly.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5101
Illegal EcoRI site found at 5542
Illegal EcoRI site found at 5973
Illegal EcoRI site found at 6414
Illegal XbaI site found at 1
Illegal SpeI site found at 266
Illegal PstI site found at 3968 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5101
Illegal EcoRI site found at 5542
Illegal EcoRI site found at 5973
Illegal EcoRI site found at 6414
Illegal SpeI site found at 266
Illegal PstI site found at 3968
Illegal NotI site found at 2797 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5101
Illegal EcoRI site found at 5542
Illegal EcoRI site found at 5973
Illegal EcoRI site found at 6414 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5101
Illegal EcoRI site found at 5542
Illegal EcoRI site found at 5973
Illegal EcoRI site found at 6414
Illegal XbaI site found at 1
Illegal SpeI site found at 266
Illegal PstI site found at 3968 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5101
Illegal EcoRI site found at 5542
Illegal EcoRI site found at 5973
Illegal EcoRI site found at 6414
Illegal XbaI site found at 1
Illegal SpeI site found at 266
Illegal PstI site found at 3968 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4658
Illegal SapI.rc site found at 4505
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Categories
Parameters
None |