Part:BBa_K5452116
5_UTR_RISC_F
Forward primer for amplifying part BBa_K5452105, allowing for a follow-up PCR reaction for different cloning strategies, such as cloning into different devices, plasmids, adding restriction sites or overhangs.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
The RNA interference (RNAi) mechanism involves key components such as Argonaute and Dicer proteins, which form a complex responsible for RNA silencing. Dicer processes double-stranded RNA (dsRNA) into small RNA duplexes of 21 to 26 nucleotides, essential for RNAi. Argonaute, containing the PAZ and Piwi domains, is part of the RNA-induced silencing complex (RISC) and appears to cleave target messenger RNAs in the RNAi pathway. In fungi, RNA-dependent RNA polymerase (RdRP) participates in the process by converting "defective" RNA into dsRNA.
RNA silencing pathways are associated with small RNAs (sRNAs) that guide proteins to target RNAs, triggering silencing. These sRNAs include siRNAs, miRNAs, and piRNAs. In fungi, siRNAs and miRNAs are derived from dsRNA but differ in origin and mechanisms. siRNAs originate from exogenous dsRNA or repetitive endogenous transcripts, while miRNAs come from genes that form single-stranded hairpin structures. Additionally, siRNAs usually perfectly match their mRNA targets, while miRNAs can have partial complementarity.
In fungi, the RNAi pathway has remained stable throughout evolution. In Schizosaccharomyces pombe, a single copy of Argonaute, Dicer, and RdRP is involved in transcriptional silencing via chromatin remodeling. In contrast, Saccharomyces cerevisiae lacks genes for RNAi, while other yeasts, such as Candida albicans, have Argonaute genes but lack Dicer or RdRP, suggesting possible non-canonical RNAi pathways or the loss of RNAi.
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