Coding

Part:BBa_K5452102

Designed by: Bianca Henriques de Oliveira   Group: iGEM24_USP-EEL-Brazil   (2024-09-27)


Dicer

his part codes for Dicer, a enzyme that belongs to the RNase III family of nucleases that process long dsRNA into 21- to 26-nucleotide (nt)-long RNA duplexes, which are fundamental specificity determinants in the RNA interference mechanism


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 570
    Illegal PstI site found at 844
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 844
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1751
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 570
    Illegal PstI site found at 844
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 570
    Illegal PstI site found at 844
    Illegal AgeI site found at 1536
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None