Composite

Part:BBa_K545042

Designed by: Grenoble 2011   Group: iGEM11_Grenoble   (2011-09-20)


Toggle Switch (LacI - TetR) which express GFP in one side

it's a Toggle Switch. Two repressors inhibits the other. the TetR side express the GFP too.

We have pTet promoter that regulate LacI gene, follow by two terminators and finally pLac promoter regulate TetR and GFP genes.

TetR repress the pTet promoter and be inhibited by (anhydro-)tetracycline. LacI repress the pLac promoter and be inhibited by IPTG (or Lactose)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2815


[edit]
Categories
Parameters
None