Measurement

Part:BBa_K545010

Designed by: Grenoble 2011   Group: iGEM11_Grenoble   (2011-09-21)


pConst + fha RBS + GFP

The production of the GFP RNA is constitutively activated. The production of the GFP protein depends on the fha RBS, inhibited by the rsmA repressor. Fixing the rsmA protein, the rsmY iRNA allow the production of the GFP protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


[edit]
Categories
Parameters
None