Part:BBa_K5443016

RBS1- TAL-Sam8

his enzyme catalyzes the deamination of L-tyrosine to 4-coumaric acid, which is the first step in the conversion of tyrosine to vanillin. This reaction is critical as it initiates the entry of tyrosine into the phenylpropanoid pathway. Reference: 1. Jendresen CB, Stahlhut SG, Li M, Gaspar P, Siedler S, Forster J, Maury J, Borodina I, Nielsen AT. Highly Active and Specific Tyrosine Ammonia-Lyases from Diverse Origins Enable Enhanced Production of Aromatic Compounds in Bacteria and Saccharomyces cerevisiae. Appl. Environ. Microbiol. 81 (13), 4458-4476 (2015). PubMed ID: 25911487 2. Jendresen CB, Stahlhut SG, Li M, Gaspar P, Siedler S, Forster J, Maury J, Borodina I, Nielsen AT. Direct Submission. Submitted (14-APR-2015) Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle, Horsholm 2970, Denmark.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 557
  Illegal PstI site found at 1397
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 557
  Illegal PstI site found at 1397
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1128
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 557
  Illegal PstI site found at 1397
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 557
  Illegal PstI site found at 1397
  Illegal AgeI site found at 502
  Illegal AgeI site found at 1113
  
• 1000
  COMPATIBLE WITH RFC[1000]