Generator

Part:BBa_K543015

Designed by: Naoto Shimada   Group: iGEM11_Tokyo_Metropolitan   (2011-09-29)

TetR generator (J23100-B0031-R0040-B0015)

TetR generator (J23100-B0031-R0040-B0015).

Tokyo Metropolitan Killing2.jpg

Repress expression of GFP by Anti-killing device

We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. we confirmed Anti-killing Device represseｓ GFP expression weakly.
Need more experience to get quantitative date.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None