Part:BBa_K542011

Catechol 2,3-dioxygenase with C-term Arg-tag (xylE-Arg)

The xylE gene was PCR'd out of genomic DNA provided by David Lain Houston* with primers adding [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#BBF_RFC_23_.28aka_Biofusion.2C_Silver_lab.29 Silver Lab Biofusion Standard] prefix/suffix. A C-terminal arginine tail (BBa_K249005) was then added.

Based on [http://2010.igem.org/Team:Lethbridge/Results#Results_2 characterization on Arg-tagged fluorescent proteins] done by the Lethbridge 2010 team, it was decided that the Arg-tag on xylE would be C-terminal.

David Lain Houston*, PhD Student
Institute of Cellular, Molecular, and Systems Biology
Susan Rosser Group
Arnott Lab
University of Glasgow

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 311
  Illegal NgoMIV site found at 483
  Illegal AgeI site found at 834
  
• 1000
  COMPATIBLE WITH RFC[1000]