Plasmid

Part:BBa_K541800

Designed by: Mustafa Elitok   Group: iGEM11_FAGEM   (2011-09-21)

B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli

This episomal vector has within its BioBrick region a constitutively expressed RFP gene in E.coli. It uses LacI promoter, B0034, mRFP1 (E0010) and B0015.

We have improved 2008 Cambridge shuttle vector as inserting E.coli expressible RFP to select white colonies at the end of clonings rather than red ones

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5187
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5187
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5193
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5187
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5187
    Illegal XbaI site found at 5202
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5997
    Illegal AgeI site found at 6109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1533
    Illegal BsaI.rc site found at 4261
    Illegal SapI site found at 3178


[edit]
Categories
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
Parameters
None