Composite

Part:BBa_K539741

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Taiwan   (2011-09-22)

TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_J61048)

The coding sequence of kivd is from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. Kivd plays the vital rule in the isobutanol biosynthesis pathway. This part kivd is under TetR repressible promoter that constitutively ON and repressed by TetRcan. And terminator(BBa_J61048) is included.

Following is the isobutanol biosythesis pathway.


Pathway%28small%29.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1735
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None